Hippocampus is an important part of the limbic system, involved in episodic memory, spatial cognition and other processes. The authors created the first single-nucleus transcriptomic atlas of the dog hippocampus using SPLiT-seq, snRNA-seq data of the hippocampus of 5-month-old Beagle dogs were obtained. After detailed preprocessing and filterin, the authors created a digital expression matrix of 105,057 single nuclei with a median of 804 genes and 1,109 counts per nucleus. Based on the differentially expressed genes (DEGs) (P-value < 0.001 and log2(fold change) > 0.25) in each cell cluster, authors identified eight cell types. You can see the UMAP result of these cell clusters below, and you can search by gene names to see the expression profile of each gene in different cell clusters.
This work was published in National Science Review at 2022 by Zhou et al. (https://doi.org/10.1093/nsr/nwac147).
